Isolation and characterization of 11 microsatellite loci in the mangrove crab, Ucides cordatus (Ocypodidae: Ucidinae) |
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Authors: | Varela Eduardo Sousa Gonçalves Evonnildo DA Costa Santos Melissa DE Oliveira Sampaio Iracilda Schneider Horacio |
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Institution: | 1. Instituto de Estudos Costeiros (IECOS), Campus de Bragan?a, UFPA, 68600‐000 Bragan?a, PA, Brazil;2. Laboratório de Polimorfismo de DNA, Departamento de Genética, UFPA, 66075‐110 Belém, PA, Brazil;3. Centro de Biologia Molecular e Engenharia Genética (CBMEG), UNICAMP, 13083‐970 Campinas, SP, Brazil |
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Abstract: | Eleven polymorphic microsatellite loci were described for the mangrove crab, Ucides cordatus, an important fishery resource on the Brazilian coast. The number of alleles observed at each locus varied between eight and 23. Observed and expected mean heterozygosities were 0.791 and 0.893 respectively. Amplification of all loci was highly successful, under the same polymerase chain reaction conditions. With the exception of P2D3, all loci adhered to the assumptions of the Hardy-Weinberg equilibrium and did not present deviations reflecting linkage disequilibrium. Given this, these markers will be extremely useful in future management programmes for U. cordatus stocks. |
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Keywords: | mangrove crab microsatellites polymorphism Ucides cordatus |
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