Characterization of 35 novel microsatellite loci for ecological and evolutionary studies of the bluntnose minnow (Pimephales notatus) |
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| Authors: | Landis J B Grose M J Wiley E O Hudman S P |
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| Institution: | Department of Ecology and Evolutionary Biology Natural History Museum and Biodiversity Institute, University of Kansas, 1345 Jayhawk Boulevard, Lawrence, KS 66045, USA Department of Biology, Truman State University, Kirksville, MO 63501, USA. |
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| Abstract: | The bluntnose minnow (Pimephales notatus) is abundant and widespread with a broad ecological niche. This species is also sexually dimorphic with strong competition between males during the mating season. We developed two genomic libraries enriched for microsatellite repeats - one dinucleotide and one tetranucleotide - and isolated 48 putative, novel microsatellite loci. Of those, we present 35 polymorphic and statistically independent microsatellite markers with two to 18 alleles per locus and heterozygosities ranging from 0.04 to 1. These markers will be useful for future behavioural, ecological, evolutionary and mating system studies using P. notatus. |
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| Keywords: | bluntnose minnow microsatellite parentage assignment Pimephales notatus population genetics |
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