Isolation and characterization of polymorphic microsatellites in the Potato Tuber Moth Tecia solanivora (Povolny, 1973) (Lepidoptera: Gelechiidae) |
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| Authors: | Torres-Leguizamon M Solignac M Vautrin D Capdevielle-Dulac C Dupas S Silvain J-F |
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| Institution: | 1. Institut de Recherche pour le Développement (IRD), UR072 Laboratoire Evolution, Génomes et Spéciation, UPR 9034, Centre National de la Recherche Scientifique (CNRS), 91198 Gif‐sur‐Yvette Cedex, France et Université Paris‐Sud 11, 91405 Orsay Cedex, France;2. Laboratoire Evolution, Génomes et Spéciation, UPR 9034, CNRS, 91198 Gif‐sur‐Yvette Cedex, France et Université Paris‐Sud 11, 91405 Orsay Cedex, France;3. Pontificia Universidad Catolica del Ecuador, Facultad de Ciencias Naturales y Biologicas, Whymper 442 y Coru?a, Quito, Ecuador |
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| Abstract: | Nine polymorphic microsatellite markers were isolated from Tecia solanivora, one of the most serious pests of potato tubers in Central and South America. As found in other studies of Lepidoptera, development of microsatellites is a difficult task: in our case, despite the large number of clones sequenced (796), of which 70 were unique, only nine loci were found to be both variable, and in Hardy-Weinberg equilibrium, No null alleles were detected. The loci were tested in three other co-occurring Gelechiidae species, one of which was variable. These loci will be used to provide a greater understanding of the genetic changes occurring during the invasive process in this species. |
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| Keywords: | Gelechiidae invasive species Lepidoptera microsatellites population genetics |
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