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Eighteen microsatellite loci in Salix arbutifolia (Salicaceae) and cross-species amplification in Salix and Populus species
Authors:Hoshikawa Takeshi  Kikuchi Satoshi  Nagamitsu Teruyoshi  Tomaru Nobuhiro
Affiliation:Laboratory of Forest Ecology and Physiology, Graduate School of Bio-agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan, Forestry and Forest Products Research Institute (FFPRI), Tsukuba 305-8687, Japan, Hokkaido Research Center, Forestry and Forest Products Research Institute (FFPRI), Sapporo 062-8516, Japan.
Abstract:Salix arbutifolia is a riparian dioecious tree species that is of conservation concern in Japan because of its highly restricted distribution. Eighteen polymorphic loci of dinucleotide microsatellites were isolated and characterized. Among these, estimates of the expected heterozygosity ranged from 0.350 to 0.879. Cross-species amplification was successful at 9-13 loci among six Salix species and at three loci in one Populus species.
Keywords:cross‐species amplification  microsatellite  riparian tree  Salicaceae  Salix arbutifolia
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