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Isolation and characterization of microsatellite loci in the plant-ant Pseudomyrmex ferrugineus (Formicidae: Pseudomyrmecinae) and cross-testing for two congeneric species
Authors:Kautz Stefanie  Schmid Volker S  Trindl Andreas  Heinze Jürgen  Ballhorn Daniel J  Heil Martin
Affiliation:1. Department of General Botany — Plant Ecology, Universit?t Duisburg‐Essen, FB BioGeo, Universit?tsstra?e 5, D‐45117 Essen, Germany;2. Biologie I, Universit?t Regensburg, Universit?tsstra?e 31, D‐93043 Regensburg, Germany;3. Departamento de Ingeniería Genética, CINVESTAV — Irapuato, Km. 9.6 Libramiento Norte, Carretera Irapuato‐León, 36821 Irapuato, Guanajuato, Mexico
Abstract:To investigate the population structure of the obligate plant-ant Pseudomyrmex ferrugineus, we developed primers for 12 microsatellite loci. We tested the variability of the markers on 11 individuals from each of two populations (totalling 22 individuals) and found two to 12 alleles per locus and population. No deviations from Hardy-Weinberg equilibrium were detected. Observed and expected heterozygosities at each locus ranged from 0.00 to 0.50 and from 0.08 to 0.46, respectively. We also investigated suitability of these primers in two congeneric species.
Keywords:Hymenoptera  mutualism  obligate Acacia‐ant  Pseudomyrmex mixtecus  Pseudomyrmex peperi  social insects
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