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Isolation and characterization of polymorphic microsatellite loci for the dace complex: Leuciscus leuciscus (Teleostei: Cyprinidae) |
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| Authors: | Dubut Vincent Martin Jean-François Gilles André VAN Houdt Jeroen Chappaz Rémi Costedoat Caroline |
| Institution: | Aix-Marseille Université, CNRS, IRD, UMR 6116 - IMEP, Equipe Persistance et Evolution de la Biodiversité, Centre Saint Charles, Case 36, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France, Montpellier SupAgro, Centre de Biologie et de Gestion des Populations, Campus International de Baillarguet, CS30016, 34988 Montferrier-sur-Lez, France, Laboratory of Animal Diversity and Systematics, Katholieke Universiteit Leuven, Charles de Bériotstraat 32, 3000 Leuven, Belgium. |
| Abstract: | Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), which is a European cyprinid species. Four multiplex polymerase chain reaction sets were optimized in order to genotype 26 polymorphic loci in all, including 16 previously published cyprinid-specific loci. The level of genetic diversity was assessed in 142 dace individuals. We also successfully applied 26 of the microsatellites to 10 related species. These primers thus will be useful to assess population structure of the dace and other cyprinid species, with application for conservation issues and phylogeographical approaches. |
| Keywords: | conservation cross‐species amplification Cyprinidae enriched library microsatellites multiplex PCR |
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