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Microsatellite markers for the tetraploid halophyte Suaeda maritima (L.) Dumort. (Chenopodiaceae) and cross-species amplification in related taxa
Authors:Prinz Kathleen  Hensen Isabell  Schie Stephan  Debener Thomas  Weising Kurt
Affiliation:Plant Molecular Systematics, University of Kassel, D-34109 Kassel, Germany, Institute of Biology/Geobotany and Botanical Garden, Martin-Luther-University Halle-Wittenberg, D-06108 Halle/Saale, Germany, Institute for Plant Genetics, University of Hannover, D-30419 Hannover, Germany.
Abstract:We developed 12 polymorphic microsatellite markers for the tetraploid halophyte Suaeda maritima (Chenopodiaceae). Population genetic parameters were estimated for three populations from different habitats (coastal and inland), using the program Tetrasat. Between two and 15 alleles per locus were observed. Mean expected heterozygosities (H(E) ) and Shannon-Wiener Diversity Indices (H') per locus and population ranged from zero to 0.852, and from zero to 2.990, respectively. The two inland populations were less diverse than the coastal one at most of the loci. All markers cross-amplified in the closely related Suaeda salsa, and all but one were transferable to Suaeda spicata and Suaeda salinaria.
Keywords:halophytes  inland salt habitats  microsatellites  SSRs  Suaeda maritima  tetraploid
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