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Imaging Protein-protein Interactions in vivo
Authors:Tom Seegar  William Barton
Institution:Biochemistry and Molecular Biology, Virginia Commonwealth University
Abstract:Protein-protein interactions are a hallmark of all essential cellular processes. However, many of these interactions are transient, or energetically weak, preventing their identification and analysis through traditional biochemical methods such as co-immunoprecipitation. In this regard, the genetically encodable fluorescent proteins (GFP, RFP, etc.) and their associated overlapping fluorescence spectrum have revolutionized our ability to monitor weak interactions in vivo using Förster resonance energy transfer (FRET)1-3. Here, we detail our use of a FRET-based proximity assay for monitoring receptor-receptor interactions on the endothelial cell surface.
Keywords:Cellular Biology  Issue 44  Förster resonance energy transfer (FRET)  confocal microscopy  angiogenesis  fluorescent proteins  protein interactions  receptors
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