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Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves) |
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| Authors: | Verkuil Yvonne I Trimbos Krijn Haddrath Oliver Baker Allan J Piersma Theunis |
| Affiliation: | Animal Ecology Group, Centre of Ecological and Evolutionary Studies (CEES), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands Department of Natural History, Royal Ontario Museum, 100 Queen's Park Crescent, Toronto, Ontario, Canada M5S 2C6 Institute for Environmental Science Leiden (CML), University of Leiden, Einsteinweg 2, 2333 CC Leiden, The Netherlands Ecology & Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada M5S 1A1 Department of Marine Ecology and Evolution, Royal Netherlands Institute for Sea Research (NIOZ), PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. |
| Abstract: | We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica). |
| Keywords: | black‐tailed godwit ground‐nesting shorebird Limosa limosa microsatellite population genetics |
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