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Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae)
Authors:Lopes Denilce Meneses  DA Silva Filipe Oliveira  Fernandes Salomão Tânia Maria  Campos Lúcio Antônio DE Oliveira  Tavares Mara Garcia
Affiliation:Departamento de Biologia Geral, Universidade Federal de Vi?osa, Vi?osa, MG, Brazil
Abstract:Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris. These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata, M. bicolor, M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees.
Keywords:ISSR  Melipona  microsatellite  population genetics  stingless bees
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