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Highly polymorphic in silico-derived microsatellite loci in the potato-infecting fungal pathogen Rhizoctonia solani anastomosis group 3 from the Colombian Andes
Authors:Ferrucho R L  Zala M  Zhang Z  Cubeta M A  Garcia-Dominguez C  Ceresini P C
Affiliation:Facultad de Agronomía, Universidad Nacional de Colombia, Carrera 30 45-03 Bogota, Colombia, Plant Pathology, Institute of Integrative Biology,ETH Zurich - Swiss Federal Institute of Technology, Universitaetstrasse 2, LFW B28, 8092, Zurich, Switzerland, Department Plant Pathology, North Carolina State University, Box 7616, Raleigh, NC 27695-7616, USA, Departamento de Fitossanidade, Engenharia Rural e Solos, UNESP - Universidade Estadual Paulista, Campus de Ilha Solteira, 15385-000 Ilha Solteira, SP, Brazil.
Abstract:Fourteen polymorphic microsatellite DNA markers derived from the draft genome sequence of Rhizoctonia solani anastomosis group 3 (AG-3), strain Rhs 1AP, were designed and characterized from the potato-infecting soil fungus R. solani AG-3. All loci were polymorphic in two field populations collected from Solanum tuberosum and S. phureja in the Colombian Andes. The total number of alleles per locus ranged from two to seven, while gene diversity (expected heterozygosity) varied from 0.11 to 0.81. Considering the variable levels of genetic diversity observed, these markers should be useful for population genetic analyses of this important dikaryotic fungal pathogen on a global scale.
Keywords:black scurf  microsatellite  population genetics  S. phureja  S. tuberosum  stem canker  Thanatephorus cucumeris
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