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Development of microsatellite markers for the dwarf bamboo species Sasa cernua and Sasa kurilensis (Poaceae) in northern Japan |
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| Authors: | Kitamura Keiko Saitoh Tomoyuki Matsuo Ayumi Suyama Yoshihisa |
| Institution: | Forest Dynamics and Diversity Group, Hokkaido Research Center, Forestry and Forest Products Research Institute, Sapporo 062-8516, Japan Kiso Experiment Station, Forestry and Forest Products Research Institute, 5473-8 Fukushima, Kiso, Nagano 397-0001, Japan Field Science Center, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, Japan. |
| Abstract: | Ten polymorphic microsatellite markers were isolated from the dwarf bamboo species Sasa cernua and Sasa kurilensis. The applicability of these markers was confirmed by genotyping of open‐pollinated seeds and leaf samples from natural populations. Genotypes of seeds from each culm shared at least one allele from the maternal parent without contradiction. All 10 loci were polymorphic in S. cernua with 2–15 alleles (average HE = 0.532). Eight loci were polymorphic in S. kurilensis with 2–10 alleles (average HE = 0.532). These markers will be useful in detailing the extent of clonal and sexual reproduction in these species. |
| Keywords: | dwarf bamboo genet identification Sasa microsatellite |
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