Isolation and characterization of microsatellites in the harlequin ladybird, Harmonia axyridis (Coleoptera, Coccinellidae), and cross-species amplification within the family Coccinellidae |
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| Authors: | Loiseau Anne Malausa Thibaut Lombaert Eric Martin Jean-François Estoup Arnaud |
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| Institution: | INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France INRA, UMR IBSV (INRA/UNSA/CNRS), 400 route des Chappes, BP 167 06903 Sophia-Antipolis Cedex, France Montpellier SupAgro, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, France. |
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| Abstract: | A total of 18 microsatellite DNA loci were isolated and characterized from the harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae). We optimized a multiplex panel consisting of two polymerase chain reactions, allowing the genotyping of all loci. The number of alleles and heterozygosity observed at each locus ranged from 1 to 12 and from 0 to 100%, respectively. After Bonferroni correction for multiple tests, none of the loci deviated significantly from Hardy-Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. Successful cross-species amplification was obtained for only three of the seven tested species of Coccinellidae. |
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| Keywords: | Biological invasion Insect Invasive Species Microsatellites Nuclear marker |
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