Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing |
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| Authors: | B Bianco A Diaspro |
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| Institution: | Department of Biophysical and Electronic Engineering, Genova, Italy. |
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| Abstract: | The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique. Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed. |
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