一株茶碱降解菌的分离和鉴定

从制药废水生物处理系统的活性污泥中经富集分离到一株能以茶碱作为唯一碳、氮源生长的茶碱降解菌Tcn3，该菌株可以利用茶碱的最高浓度为3 000 mg/L。当茶碱浓度为1000 mg/L时被彻底降解的时间仅需48 h。Tcn3菌株降解茶碱的最适pH为80。K＋是该菌株降解茶碱的必需元素。采用16S rDNA序列分析法及传统的生理生化特征鉴定法对该菌株进行鉴定，结果表明，Tcn3的16S rDNA的核苷酸序列与善变副球菌(Paracoccus versutus) ATCC 25364的同源性为997%，在细菌系统发育分类学上属于变形菌α亚类，Rhodobacter组：副球菌属，善变副球菌。

Isolation and Identification of a Theophylline-degrading Bacterial Strain

Bacterial strain Tcn3 was isolated from the activated sludge of a pharmaceutical wastewater treatment system, which could grow with theophylline as the sole source of carbon and energy. The maximum concentration of theophylline degraded by Tcn3 is 3 000mg/L. When the concentration of theoplyline is 1 000mg/L, a complete degradation only needs 48h. For Tcn3 degrading theophylline, the optimal pH is 8^0 and K++ is the necessary element. The sequence analysis of a 1 500bp 16SrDNA fragment amplified from total DNA of strain Tcn3 and the traditional identification methods was used to identify Tcn3. The results showed that Tcn3 shares 99.7% 16S rDNA sequence homology with Paracoccus versutus type strain ATCC 25364. In phylogenetic framework of bacterial classification, Tcn3 belongs to proteobacteria; alpha subdivision; Rhodobacter group: Paracoccus versutus.