Histological analysis reveals the formation of shoots rather than embryos in regenerating cultures of <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> |
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| Authors: | Izabela Dobrowolska Gisele M Andrade David Clapham Ulrika Egertsdotter |
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| Institution: | 1.Department of Forest Genetics and Plant Physiology,Ume? Plant Science Center, Swedish University of Agricultural Sciences,Ume?,Sweden;2.Department of Cell Biology, Faculty of Biology and Environment Protection,University of Silesia,Katowice,Poland;3.Department of Plant Biology and Forest Genetics,Uppsala Biocenter, Swedish University of Agricultural Sciences,Uppsala,Sweden;4.G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology,Atlanta,USA |
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| Abstract: | Eucalyptus globulus is an important species in international forestry in regions with Mediterranean climates and comprises 65?% of the plantation hardwood in Australia. Propagation by somatic embryogenesis would offer many advantages and its development has received much attention. Structures regenerating on explants from hypocotyls of mature zygotic embryos of E. globulus cultured on medium with NAA, reported previously to be effective for embryogenic regeneration, were analyzed morphologically and histologically to clarify their pathway of development. Analysis of series of sections revealed organogenic, rather than embryogenic, pathways of regeneration in this system. We show that protocols for propagation of E. globulus must be carefully evaluated by microscopic examination of adequate numbers of serial sections. |
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