Effect of boron on microspore embryogenesis and direct embryo to plant conversion in Brassica napus (L.) |
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| Authors: | Pitchayapa Mahasuk Annika Stina Kullik Mohammed Cassim Iqbal Christian Möllers |
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| Affiliation: | 1.Department of Crop Sciences,University of G?ttingen,G?ttingen,Germany;2.Plant and Environmental Sciences,National Institute of Fundamental Studies,Kandy,Sri Lanka |
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| Abstract: | The NLN-medium has been successfully used, since 1982, for microspore culture in Brassica napus and other Brassica species. Changes to the media composition were restricted to carbohydrate and nitrogen sources and growth regulators while micro-nutrients have not been optimized. The NLN-medium contains boron at a concentration of 162 µM. Boron is required for diverse physiological and metabolic processes in the cell. This study investigated the effect of seven- and 13-fold increased boron concentration on the induction of embryos in microspore cultures of four genotypes of B. napus. A significant improvement of microspore embryogenesis was achieved by both enhanced boron concentrations in the NLN medium. No effect on the regeneration of embryo to plant conversion was observed. |
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