首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Ion-exchange properties of the cell walls isolated from suspension-cultured plant cells
Authors:Nataly Meychik  Yuliya Nikolaeva  Maria Kushunina  Maria Titova  Alexander Nosov
Institution:1.Department of Plant Physiology, Faculty of Biology,Moscow State University,Moscow,Russia;2.Timiryazev Institute of Plant Physiology,Russian Academy of Sciences,Moscow,Russia
Abstract:Ion-exchange capacity of the cell walls isolated from suspension-cultured Panax japonicus, Polyscias filicifolia and Dioscorea deltoidea cells was analyzed at pH 2.8–12 and constant ionic strength (100 mM). The cell walls of all cultures contain three types of ion-exchange groups: primary amino groups (pK a < 3), carboxyl groups of polygalacturonic acid (pK a 3.71), and carboxyl groups of hydroxycinnamic acids (pK a 7.62). Amount of primary amino groups ranges from 500 (D. deltoidea) to 710 (P. japonicus) µmol/g cell wall dry weight, carboxyl groups with pK a 3.71—from 570 (D. deltoidea) to 670 (P. filicifolia), carboxyl groups with pK a 7.62—from 270 (P. filicifolia) to 370 (P. japonicus) µmol/g cell wall dry weight. The comparison of the data obtained by elemental and functional analyses demonstrated that the cell walls of all cultures are characterized by high content of pectins (~40% by weight) and structural proteins (~17–30% by weight), but do not contain phenolic OH–groups, which presumably signifies the absence of lignin in them.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号