Construction of an ∼2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2 |
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| Authors: | Ming Li Wang Lu Huang Deverie K Bongard-Pierce Stephen Belmonte Eve Ann Zachgo John W Morris Maureen Dolan Howard M Goodman |
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| Institution: | Department of Genetics, Harvard Medical School Massachusetts General Hospital, Boston, MA 02114, USA,;Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA |
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| Abstract: | A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artifical chromosome (YAC) physical map is described. An ～2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing. |
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