Absorbance-temperature profile of ribosomes from seeds of Pinus lambertiana |
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Authors: | T S Ko |
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Institution: | Department of Biochemistry and Nutrition Virginia Polytechnic Institute and State University, Blacksburg, Va. 24061 USA |
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Abstract: | The absorbance (260 nm)-temperature profile of ribosomes from seeds of , in the temperature range of about 25 – 60° was observed to be biphasic. When r-proteins in ribosomes were dansylated by 5-dimethylamino-1-naphthalenesulfonyl chloride dispersed on celite (180 molecules of 5-dimethylamino-1-naphthalenesulfonyl chloride per one ribosome particle), each transition midpoint was lowered by 2 – 3° and the sharpness of the transition of each phase was increased. The result of the present work suggests discrete cooperative transitions of the conformation of r-RNAs in ribosomes and its control by r-proteins. |
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