Biofilm formation in aqueous metal working fluids |
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| Institution: | 1. Department of Renewable Resources, University of Alberta, Edmonton, Alberta T6G 2H1, Canada;2. School of Land Science and Technology, China University of Geosciences (Beijing), Beijing 100083, China;1. Department of Cardiac Surgery, Erasme Hospital, Free University of Brussels, Brussels, Belgium;2. Department of Cardiac Surgery, University Hospital of Ghent, Ghent, Belgium;3. Laboratory of Experimental Cardiac Surgery, Ghent University, Ghent, Belgium;1. Division of Cardiology;2. Department of Medicine;3. Department of Cardiothoracic and Vascular Surgery, Montefiore Medical Center, Albert Einstein College of Medicine, New York, New York;1. School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore, Singapore;2. Key Laboratory of New Technology for Construction of Cities in Mountain Area (Chongqing University), Ministry of Education, Chongqing 400045, PR China;3. School of Civil Engineering, Chongqing University, Chongqing 400045, PR China |
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| Abstract: | Planktonic and biofilm flora in circulating coolant systems were examined in engineering workshops and in a laboratory model. In a CNC lathe, thorough cleaning and disinfection reduced microbial counts, but within five weeks substantail numbers of organism were detected in both the coolant and, especially, the biofilm. Physical disruption of the biofilm in a milling machine, using a wire brush, increased the planktonic bacterial counts up to twenty times. Results from the model system indicate that biocides may increase the time before the development of high microbial numbers, both in the biofilm and the coolant. The use of a metal-working fluid containing a triazine biocide was shown to result in the development of substantial fungal contamination with most being associated with the biofilm. |
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