Solution properties of high-molar-mass hyaluronans: the biopolymer degradation by ascorbate |
| |
Authors: | Soltés Ladislav Valachová Katarína Mendichi Raniero Kogan Grigorij Arnhold Juergen Gemeiner Peter |
| |
Institution: | Institute of Experimental Pharmacology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia. ladislav.soltes@savba.sk |
| |
Abstract: | An accurate molecular characterization, molar mass and size distributions, of 10 hyaluronan (HA) samples was performed by using a multi-angle light scattering detector connected on-line to a size exclusion chromatographic system. The dynamic viscosity eta of the HA solutions was investigated using a rotational viscometer. On monitoring the sample dynamic viscosity for up to 5h, a small however constant increase of the eta value was observed, indicating rheopectic behavior of all 10 HA solutions. Addition of ascorbic acid to the HA solutions caused significant changes in the rheological properties of the samples investigated. The change of eta values in the course of time was explained by the redox reactions (caused by the added ascorbate) that occur during the dynamic viscosity monitoring. |
| |
Keywords: | Hyaluronan degradation Rotational viscometry Ascorbic acid |
本文献已被 ScienceDirect PubMed 等数据库收录! |