Cloning and characterization of a cathepsin D inhibitor gene from Solanum tuberosum L. |
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Authors: | Karin Herbers Salomé Prat Lothar Willmitzer |
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Affiliation: | (1) Institut für Genbiologische Forschung GmbH, Ihnestrasse 63, 14195 Berlin, Germany;(2) Present address: Abt. Molekulare Pflanzenphysiologie, Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gaterleben, Germany;(3) Present address: Centro de Investigacion y Desarrollo, Jordi Girona, 18-26, 08034 Barcelona, Spain |
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Abstract: | A DNA clone encoding a cathepsin D inhibitor CathInh was isolated from a potato genomic library using a CathInh cDNA as hybridization probe. The amino acid sequence of the coding region is nearly identical with a CathInh cDNA and CathInh proteins previously isolated from a tuber-specific cDNA library and from tubers, respectively. Analysis of GUS activity resulting from expression of chimeric CathInh promoter-GUS genes in transgenic potato plants revealed expression exclusively confined to potato tubers. No GUS activity could be detected in any other organ of the transgenic plants either constitutively or after wounding or treatment with abscisic and jasmonic acid (JA). Interestingly, part of the promoter region of the CathInh gene, essential for GUS activity in tubers, shows striking similarity to promoter regions of tuber-specific class I patatin genes. |
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Keywords: | cathepsin D inhibitor gene promoter analysis Solanum tuberosum tuber-specific expression |
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