Transgenic plants of turfgrass (Agrostis palustris Huds.) from microprojectile bombardment of embryogenic callus |
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| Authors: | Heng Zhong Mark G Bolyard C Srinivasan Mariam B Sticklen |
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| Institution: | (1) Departments of Crop and Soil Sciences and of Entomology, 202 Pesticide Research Center, Michigan State University, 48824-1311 East Lansing, MI, USA;(2) Present address: Department of Biological Sciences, SL 3328 Science Building, Southern Illinois University at Edwardsville, 62026-1651 Edwardsville, IL, USA;(3) Present address: Department of Microbiology, S126, Michigan State University, 48824 East Lansing, MI, USA |
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| Abstract: | Transgenic creeping bentgrass (Agrostis palustris Huds., cv. Pencross; Poaceae) plants have been obtained by microprojectile bombardment of and regeneration from embryogenic calli with a vector designed to deliver the  -glucuronidase (GUS) gene under the control of rice actin 1 5' regulatory sequences. Southern analysis of polymerase chain reaction (PCR)-amplified and restriction-digested genomic DNA of four transgenic plants regenerated from these cultures showed the unscrambled integration of the gus fragment. Northern blot analysis confirmed the expression of gus mRNA in one of the transgenic plants. Western blot analysis revealed a high level of accumulation of gus protein. Histochemical assays showed enzymatic activity of -glucuronidase in all parts of the transgenic turfgrass plant. The order of gus expression level in different tissues of the transgenic plant is as follows: stem node > first young leaf > root tip > second / third / fourth young leaf > stem internode > root hair-zone.Abbreviation GUS
-glucuronidase
- MS
Murashige and Skoog(1962) medium
- BA
6-benzyladenine
- dicamba
3, 6 -dichloro-o-anisic acid
- PCR
polymerase chain reaction |
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| Keywords: | |
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