应用A-PAGE技术研究玉米自交系资源种子蛋白的遗传多样性

采用酸性聚丙烯酰胺凝胶电泳(A-PAGE)技术对260份玉米自交系种质资源的种子储藏蛋白进行检测,分析了这组种质在蛋白水平上的遗传多样性。结果表明,在供试自交系中,种子盐溶和酸溶蛋白电泳谱带均显示出较高的多态性,并存在一致性。其中盐溶蛋白电泳谱带数目多于酸溶蛋白,且95%为多态性谱带,表明盐溶蛋白在供试自交系资源中具有丰富的多样性。利用盐溶蛋白多态性完成的聚类分析结果和自交系系谱及杂种优势群之间没有明显的对应关系,表明储藏蛋白A-PAGE方法对于大规模玉米种质资源的遗传多样性分析和类群划分可能存在局限性。

Genetic Diversity of Seed Storage Proteins in Maize Germplasm by Acid Polyacrylamide Gel Electrophoresis (A-PAGE)

Seed storage proteins of 260 maize inbred lines were analyzed by acid polyacrylamide gel electrophoresis(A-PAGE) in order to clarify genetic diversity of these lines.Globulin and glutelin showed high polymorphism in electrophoretic pattern in these lines,and both were quite similar.The number of globulin bands,95% of which were polymorphic in these lines,was more than that of glutelin bands.Clustering results showed that the clusters were not corresponding to heterotic groups and pedigrees,which suggested that the A-PAGE of seed storage proteins might not be a good method for large-scale analysis of genetic diversity and heterotic grouping in maize germplasm.