Fluorescence correlation spectroscopy in the nanosecond time range: Photon antibunching in dye fluorescence |
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Authors: | P Kask P Piksarv Ü Mets |
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Institution: | (1) Institute of Chemical Physics and Biophysics, Estonian Academy of Sciences, Lenini puiestee 10, 200 001 Tallinn, USSR |
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Abstract: | Possibilities for the use of fluorescence correlation spectroscopy in the nanosecond time range are demonstrated. The experiment is based on a cw argon ion laser, a microfluorimeter, two photon detectors, and a time-to-analog converting system. Experiments using solutions of rhodamine 6G and pyronine G in water at concentrations of about 20 molecules per sample volume are reported. The photon anticorrelation component decaying with a time constant close to the excited state lifetime was observed. |
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Keywords: | Fluorescence correlation spectroscopy photon antibunching in dye fluorescence |
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