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Insolubilized coenzymes: the covalent coupling of enzymatically active NAD to glass surfaces |
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| Authors: | M K Weibel H H Weetall H J Bright |
| Affiliation: | 1. Department of Biochemistry, School of Medicine University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA;2. Research and Development Laboratory Corning Glass Works, Corning, N. Y. 14830 USA |
| Abstract: | NAD was attached to the surface of glass beads by a diazo coupling procedure. The insolubilized NAD was shown to function as a coenzyme in the yeast alcohol dehydrogenase reaction. |
| Keywords: | YADH, yeast alcohol dehydrogenase BSA, bovine serum albumin NAD, nicotinamide adenine dinucleotide |
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