Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca) |
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Authors: | David I. Dunstan Terry D. Bethune Cheryl A. Bock |
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Affiliation: | (1) Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, S7N 0W9 Saskatoon, Saskatchewan, Canada |
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Abstract: | Summary The production of cotyledonary somatic embryos of white spruce from cultures grown long-term as suspensions was investigated. We report the effects of removal of 2,4-dichlorophenoxyacetic acid (2,4-D) from the maintenance medium (ordinarily containing both 2,4-D and benzyl adenine) before (±)-ABA-stimulated maturation. In particular the use of a 1-wk culture period without 2,4-D was found to improve the production of normal-looking cotyledonary somatic embryos. Using high performance liquid chromatography analyses of culture supernatants, it was determined that this affect was not related to altered ABA metabolism. Germination of cotyledonary somatic embryos from cultures pretreated by the 1-wk culture period without 2,4-D was improved compared with similar embryos from cultures that had not been pretreated. |
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Keywords: | long-term suspension cultures white spruce somatic embryo |
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