Phosphofructokinase from porcine heart, liver and erythrocytes |
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| Authors: | T Koyama T Ema N Hashizume A Hachimori E Iizuka |
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| Institution: | Institute of High Polymer Research, Faculty of Textile Science and Technology, Shishu University, Nagano, Japan. |
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| Abstract: | 1. Phosphofructokinase from porcine heart, liver and erythrocytes were purified by affinity chromatographies on Cibacron Blue Sepharose and N6-ATP agarose. 2. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that the heart and liver enzymes consist of only one kind of subunit, namely, M and L type subunits, respectively, whereas the erythrocyte enzyme comprises of three kinds of subunits, M, L and C types. 3. Some kinetic and regulatory properties of the enzymes were also measured. |
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