Effect of Nitric Oxide Donors on GABA Uptake by Rat Brain Synaptosomes |
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Authors: | Cupello A Mainardi P Robello M Thellung S |
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Institution: | (1) Centro di Neurofisiologia Cerebrale, C.N.R., Genova, Italy;(2) Dipartimento di Fisica dell'Università, Genova, Italy |
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Abstract: | The effect of nitric oxide donors and L-arginine on the uptake of GABA was studied in synaptosomes purified from rat brain. The neurotransmitter uptake was significantly reduced by S-nitrosoacetylpenicillamine and by sodium nitroprusside, although in this case to a lesser extent. A slight inhibitory effect was found preincubating rat brain synaptosomes with 1 mM L-arginine as well. The S-nitrosoacetylpenicillamine effect gradually disappeared with decomposition of the substance by exposure to light. The nitric oxide effect appears to be mainly due to a decrease in the V for synaptosomal GABA uptake and seems to be related to a partial collapse of nerve endings ionic gradients. Functionally, it could result over time in a reduced availability of GABA at the synapses involved. |
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Keywords: | Nitric oxide GABA uptake synaptosomes |
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