Effective detergent/lipid ratios in the solubilization of phosphatidylcholine vesicles by Triton X-100. |
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Authors: | M A Partearroyo M A Urbaneja F M Go?i |
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Institution: | Department of Biochemistry, University of the Basque Country, Bilbao, Spain. |
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Abstract: | Effective detergent:lipid ratios (i.e. molar ratios in the mixed aggregates, vesicles or micelles) have been estimated for the solubilization of phosphatidylcholine vesicles by Triton X-100. Effective molar ratios are given for both the onset and the completion of bilayer solubilization; small unilamellar, large unilamellar and multilamellar vesicles have been used. Effective detergent:lipid ratios are independent of phospholipid concentration, and their use allows a deeper understanding of membrane-surfactant interactions. |
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