| 孤儿受体TR3在小鼠睾丸中的定位和表达 |
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| 引用本文： | Mu XM,Liu YX. 孤儿受体TR3在小鼠睾丸中的定位和表达[J]. 生理学报, 1998, 50(4): 439-443 |
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| 作者姓名： | Mu XM Liu YX |
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| 作者单位： | 中国科学院动物研究所生殖生物学国家重点实验室,北京,100080 |
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| 基金项目： | 中国科学院九五重点项目!KJ952-J1-603,自然科学基金!39670285,国家九五攀登预选项目 |
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| 摘 要： | 本文采用原位杂交和免疫组织化学技术,观察孤儿受体ＴＲ３及其ｍＲＮＡ在小鼠睾丸中的表达及细胞定位。结果表明,在小鼠睾丸中有显著量的孤儿受体ＴＲ３ ｍＲＮＡ和蛋白表达,其表达量在不同曲细精管有明显的差异;孤儿受体ＴＲ３蛋白主要定位于生精细胞,其ｍＲＮＡ在生精细胞特异表达,主要在精原细胞和发育早期的初级精母细胞表达,提示孤儿受体ＴＲ３在小鼠曲细精管精子发生的早期阶段中起着调控作用。
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| 关 键 词： | 孤儿受体TR3 原位杂交 免疫组织化学 精子发生 |
| 修稿时间： | 1997-07-14 |
Localization and expression of TR3 orphan receptor in mouse testis |
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| Mu X M,Liu Y X. Localization and expression of TR3 orphan receptor in mouse testis[J]. Acta Physiologica Sinica, 1998, 50(4): 439-443 |
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| Authors: | Mu X M Liu Y X |
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| Affiliation: | State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080. |
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| Abstract: | By means of in situ hybridization and immunohistochemistry, the expression and cellular localization of orphan receptor TR3 and its mRNA were observed. The results showed that both TR3 and its mRNA were expressed in a significant amount in mouse testis, and the expression level of TR3 mRNA was different in current seminiferous tubules. TR3 mRNA was specifically expressed in germ cells, mainly in spermatogonia and less advanced primay spermaaatocytes, whereas TR3 receptor protein was banlylocalized in germ cells. It is suggested that TR3 may play an important role in regulating the early stage of germ cell development in mice. |
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| Keywords: | orphan receptor TR3 in situ hybridization immunohistochemistry spermatogenesic |
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