The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 expresses the monofucosyl A type 1 histo-blood group epitope |
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| Authors: | Mario A Monteiro P.Y Zheng Ben J Appelmelk Malcolm B Perry |
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| Affiliation: | Canadian Bacterial Diseases Network, Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ont. K1A OR6, Canada;Department of Medical Microbiology, Vrije Universiteit Medical School, Amsterdam, The Netherlands;Department of Gastroenterology, 2nd Affiliated Hospital, Henan Medical University, Zhengzhou, China |
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| Abstract: | The lipopolysaccharide of Helicobacter mustelae type strain ATCC 43772 was obtained by phenol-water extraction of bacterial cells. Structural investigations were made on the lipid A free saccharide moiety released from the lipopolysaccharide by mild acetic acid hydrolysis. Nuclear magnetic resonance, gas liquid chromatography-mass spectrometry and fast atom bombardment-mass spectrometry were employed in the characterization of products from chemical manipulations. A monoclonal antibody specific for blood group A reacted strongly with lipopolysaccharide of H. mustelae. Chemical and serological data showed that the outer core region of the lipopolysaccharide from H. mustelae ATCC 43772 expresses the monofucosyl A type 1, α-d-GalNAc-(1→3)-[α-l-Fuc-(1→2]-β-d-Gal-(1→3)-β-d-GlcNAc, blood group determinant, a mimic of animal cell surface glycolipids and glycoproteins. |
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| Keywords: | Blood group A type 1 Helicobacter mustelae Lipopolysaccharide Molecular mimicry Structural determination |
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