Galactomannan from Ambiguous Crazyweed (Oxytropis ambigua(Pall.) DC) |
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Authors: | Mestechkina N. M. Lobanova I. E. Anulov O. V. Shcherbukhin V. D. |
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Affiliation: | (1) Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr., 33, Moscow, 117071, Russia;(2) Central Botanical Garden, Siberian Branch of RAS, Novosibirsk, 630090, Russia |
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Abstract: | A galactomannan with a molecular weight of 735 kDa was first isolated and purified from seeds of ambiguous crazyweed Oxytropis ambigua(Pall) DC (family Leguminosae) with a yield of 3.6%. Its aqueous solutions displayed an optical activity ([]D= 73.32°) and high viscosity ([] = 644 ml/g). Chemical analysis and 13C-NMR spectroscopy revealed the presence of D-mannopyranose and D-glucopyranose in the heteropolysaccharide at a molar ratio of 1.39 : 1. The linear backbone of its macromolecule consists of 1,4--D-mannopyranose residues. Single -D-galactose residues substitute 72% of the mannoses to form branches. |
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