Detection of antibodies to Sendai virus in mouse sera by enzyme-linked immunosorbent assay (ELISA) |
| |
| Authors: | E Suzuki A Noguchi H Takeda |
| |
| Institution: | Department of Veterinary Science, National Institute of Health, Tokyo. |
| |
| Abstract: | Enzyme-linked immunosorbent assay (ELISA) for diagnosis of Sendai virus infection in mice was evaluated. A large-scale survey of infected mice showed that ELISA is approximately 100 times more sensitive than the hemagglutination-inhibition or complement-fixation test. Although a few SPF mice showed false-positive reactions at a serum dilution of 1:40, further dilution to 1:80 eliminated the non-specific reaction. It was shown that ELISA is a highly satisfactory method for examination of Sendai virus infection in mice. |
| |
| Keywords: | |
|
|
