| TC-RT-PCR检测菜豆荚斑驳病毒的研究 |
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| 引用本文： | 沈建国,高芳銮,廖富荣,王念武,郭琼霞,吴祖建. TC-RT-PCR检测菜豆荚斑驳病毒的研究[J]. 激光生物学报, 2009, 18(1) |
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| 作者姓名： | 沈建国 高芳銮 廖富荣 王念武 郭琼霞 吴祖建 |
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| 作者单位： | 福建出入境检验检疫局技术中心,福建省检验检疫技术研究重点实验室,福建,福州,350001;福建农林大学植物病毒研究所,福建,福州,350002;福建农林大学植物病毒研究所,福建,福州,350002;厦门出入境检验检疫局,福建,厦门,361012;福建出入境检验检疫局技术中心,福建省检验检疫技术研究重点实验室,福建,福州,350001 |
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| 基金项目： | 福建省自然科学基金，福建科技重大专项专题项目，福建出入境检验检疫局科技项目 |
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| 摘 要： | 根据已报道的菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)外壳蛋白(Coat protein,CP)基因序列设计特异性引物,应用试管捕捉RT-PCR(Tube capture RT-PCR,TC-RT-PCR)技术对大豆种皮上的BPMV进行检测.结果表明,TC-RT-PCR方法能从携带BPMV的大豆种皮上扩增到预期大小的基因片段.将TC-RT-PGR扩增产物克隆测序后进行序列分析,结果显示扩增到的片段序列与BPMV的CP基因序列具有高度同源性,进一步证实了该方法的准确性.应用TC-RT-PCR方法,成功检测了一批进境大豆样品.本文建立的TC-RT-PCR方法,为大豆种子上BPMV的检测和诊断提供了一种新的参考方法.
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| 关 键 词： | 菜豆荚斑驳病毒 检测 TC-RT-PCR |
Detection of Bean pod mottle virus by TC-RT-PCR |
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| SHEN Jian-guo,GAO Fang-luan,LIAO Fu-rong,WANG Nian-wu,GUO Qiong-xia,WU Zu-jian. Detection of Bean pod mottle virus by TC-RT-PCR[J]. ACTA Laser Biology Sinica, 2009, 18(1) |
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| Authors: | SHEN Jian-guo GAO Fang-luan LIAO Fu-rong WANG Nian-wu GUO Qiong-xia WU Zu-jian |
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| Affiliation: | 1.Fujian Key Laboratory for Technology Research of Inspection and Quarantine;Technique Center of Fujian Exit-Entry Inspection and Quarantine Bureau;Fuzhou 350001;Fujian;China;2.Institute of Plant Virology;Fujian Agriculture and Forestry University;Fuzhou 350002;3.Xiamen Exit-Entry Inspection and Quarantine Bureau;Xiamen 361012;China |
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| Abstract: | A pair of specific primers were designed and synthesized based on the conserved nucleotide sequence of coat protein(CP) gene of Bean pod mottle virus(BPMV).Tube capture RT-PCR(TC-RT-PCR) was established to detect BPMV in soybean seed coat.The results showed that the target fragment could be amplified from the BPMV-infected soybean seed coats.Cloning and sequencing analysis indicated that nucleotide sequence of the target fragment was highly identical to the reported BPMV genomic sequence,which verified the ... |
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| Keywords: | TC-RT-PCR |
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