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STUDIES OF THE DETERMINATION OF THE BACTERIUM COLI TYPE I CONTENT OF FARM WATER SUPPLIES |
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| Authors: | S. B. THOMAS L. F. L. CLEGG W. A. CUTHBERT C. D. OXLEY C. A. SCARLETT C. H. WESTWATER G. ELIS JONES J. McCLEMONT C. S. MORRIS AGNES A. NICHOLS |
| Affiliation: | (Members of Water Supplies Panel of the Conference of Advisory Bacteriologists, Ministry of Agriculture) |
| Abstract: | SUMMARY: A – comparison of the suitability of brilliant green bile broth and MacConkey's broth at 44° for the detection of Bacterium coli type I in farm water supplies, showed that 83.1% of the samples had no difference in the number of positive tubes at 44°, and only 5 samples (1.7%) had a significantly higher number of positive tubes in MacConkey's broth. Of 707 strains of coli-aerogenes bacteria isolated from 44° positive tubes of both media, 94.5% were Bact. coli type I. Strains of Bact. coli type II and Bact. aerogenes type I which were 44° positive constituted 3.7% and 0.4% respectively, all of which were indole negative at 44°. In addition there were 10 strains (1.4%) of 44° positive Intermediate type II, 9 of which were indole positive at 44°. An appreciable number (6.6%) of Bact. coli type I strains failed to give a positive indole reaction in 24 hr at 44°. |
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