Identification and characterization of BGL11(t), a novel gene regulating leaf-color mutation in rice (Oryza sativa L.) |
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Authors: | Zhi-kun Wang Yun-xiang Huang Zheng-diao Miao Zhi-yan Hu Xin-zhang Song Li Liu |
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Institution: | 1. Institute of Applied Chemistry, Zhejiang Agriculture and Forestry University, Lin’an, 311300, Zhejiang, China 2. College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, Hunan, China 3. School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin’an, 311300, Zhejiang, China
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Abstract: | A novel bright-green leaf mutant, bgl11, derived from Nipponbare (Oryza sativa L. ssp. japonica) treated by ethyl methanesulfonate (EMS), exhibited a distinct bright-green leaf phenotype throughout development. Chlorophyll contents of bgl11 decreased significantly than that of its wild-type parent. Genetic analysis suggested that the bright-green leaf trait was controlled by a single recessive nuclear gene, which was tentatively designed as BGL11(t). To isolate the BGL11(t) gene, a map-based cloning strategy was employed, and the gene was finally mapped in a 94.7 kb region between marker InDel11-5 and InDel11-9 on the long arm of chromosome 11, in which no gene leaded to leaf-color mutation had been mapped or cloned. Cloning and sequencing analysis revealed that, LOC_Os11g38040, which was predicted to encode an expressed protein, had a 9 bp segment deletion in the coding region of bgl11. Furthermore, the transgenic plants with wild-type gene LOC_Os11g38040 were restored to normal phenotype. Accordingly, the gene (LOC_Os11g38040) was identified as the BGL11(t) gene. These results are very valuable for further study on BGL11(t) gene and illuminating the mechanism of chloroplast development in rice. |
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