Stability of solvent formation in Clostridium acetobutylicum during repeated subculturing |
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Authors: | Maris G. N. Hartmanis Henrik Åhlman Sten Gatenbeck |
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Affiliation: | (1) Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-10044 Stockholm, Sweden;(2) Present address: National Heart, Lung, and Blood Institue, National Institutes of Health, Building 3, Room 103, 20 892 Bethesda, Maryland, USA |
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Abstract: | Summary Clostridium acetobutylicum ATCC 824 was submitted to repeated subculturing at 24-hour intervals for 218 days. The organism retained its ability to form solvents, although the fermentation slowly became increasingly acidogenic during the first 200 days. Except for the initial spore inoculum, the cultures were not subjected to heat shocking between the serial transfers. When the inoculum volume was doubled from 3.3% to 6.7% after 200 days of subculturing, the product formation pattern quickly shifted back from acids to primarily butanol. Acetone production also resumed after being undetectable for more than 50 days. The relative formation of acetate and ethanol remained nearly constant throughout the experiments, while the formation of butyrate mirrored that of butanol. |
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