|
|||||
|
|
N 6 -( 2 -isopentenyl) adenosine: biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli |
|
| Authors: | John K Bartz Dieter Söll |
| Institution: | Department of Molecular Biophysics and Biochemistry Yale University New Haven, Connecticut 06520, USA |
| Abstract: | An enzyme has been partially purified from Escherichia coli which catalyzes in vitro the transfer of the Δ2-isopentenyl group from Δ2-isopentenyl pyrophosphate to an adenosine residue in Mycoplasma sp. (Kid) tRNA. The product of the reaction is N6-(Δ2-isopentenyl) adenosine, which is known to be absent in this Mycoplasma tRNA. The enzyme has an approximate molecular weight of 55,000 daltons, requires reduced sulfhydryl groups and a divalent metal ion for full activity, and is specific for tRNA. |
| Keywords: | iA N6-(Δ2-isopentenyl-adenosine msiA 2-methylthio-N6-(Δ2-isopentenyl)adenosine A adenosine Ap adenosine 3′-phosphate pA adenosine 5′-phosphate ψp pseudouridine 3′-phosphate Cp cytidine 3'-phosphate BD-cellulose benzoylated DEAE-cellulose |
| 本文献已被 ScienceDirect 等数据库收录！ | |