Engineered Saccharomyces cerevisiae that produces 1,3‐propanediol from d‐glucose |
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| Authors: | Z. Rao Z. Ma W. Shen H. Fang J. Zhuge X. Wang |
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| Affiliation: | The Key Laboratory of Industrial Biotechnology of Ministry of Education, Research Center of Industrial Microbiology, School of Biotechnology and State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, P.R. China |
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| Abstract: | Aims: Saccharomyces cerevisiae is a safe micro‐organism used in fermentation industry. 1,3‐Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3‐propanediol at low cost. Methods and Results: By using d ‐glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3‐propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3‐propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303‐1A by Agrobacterium tumefaciens‐mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3‐propanediol from d ‐glucose. Conclusion: Saccharomyces cerevisiae can be engineered to produce 1,3‐propanediol from low‐cost feedstock d ‐glucose. Significance and Impact of the Study: To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3‐propanediol by using A. tumefaciens‐mediated transformation. This study might lead to a safe and cost‐efficient method for industrial production of 1,3‐propanediol. |
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| Keywords: | Agrobacterium tumefaciens dhaB glycerol 1,3‐propanediol Saccharomyces cerevisiae yqhD |
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