Selective disruption of energy flow from phycobilisomes to Photosystem I |
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| Authors: | Alexander N Glazer Yvonne M Gindt Crystal F Chan Kenneth Sauer |
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| Institution: | (1) Department of Molecular and Cell Biology, University of California, 229 Stanley Hall, 94720 Berkeley, CA, USA;(2) Structural Biology Division, Lawrence Berkeley Laboratory, University of California, 94720 Berkeley, CA, USA;(3) Department of Chemistry, University of California, 94720 Berkeley, CA, USA;(4) Present address: Department of Chemistry, Michigan State University, 48824 East Lansing, MI, USA |
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| Abstract: | Efficient production of ATP and NADPH by the  light reactions of oxygen-evolving photosynthesis demands continuous adjustment of transfer of absorbed light energy from antenna complexes to Photosystem I (PS I) and II (PS II) reaction center complexes in response to changes in light quality. Treatment of intact cyanobacterial cells with N-ethylmaleimide appears to disrupt energy transfer from phycobilisomes to Photosystem I (PS I). Energy transfer from phycobilisomes to Photosystem II (PS II) is unperturbed. Spectroscopic analysis indicates that the individual complexes (phycobilisomes, PS II, PS I) remain functionally intact under these conditions. The results are consistent with the presence of connections between phycobiliproteins and both PS II and PS I, but they do not support the existence of direct contacts between the two photosystems.Abbreviations Chl
chlorophyll
- EPR
electron paramagnetic resonance
- NEM
N-ethylmaleimide
- PBS
phycobilisome
- PS
photosystem |
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| Keywords: | cyanobacteria excitation energy transfer epr fluorescence sulfhydryl modification |
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