| 长穗偃麦草酸性磷酸酶与碱性磷酸酶编码基因的染色体定位 |
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| 引用本文： | 李玉京,李继云.长穗偃麦草酸性磷酸酶与碱性磷酸酶编码基因的染色体定位[J].遗传学报,1998,25(5):449-453. |
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| 作者姓名： | 李玉京 李继云 |
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| 作者单位： | 中国科学院遗传研究所，中国科学院遗传研究所，中国科学院遗传研究所，中国科学院生态环境研究中心，中国科学院遗传研究所，中国科学院遗传研究所 北京 100101，北京 100101，北京 100101，北京 100085，北京 100101，北京 100101 |
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| 基金项目： | 国家自然科学基金重大课题(编号:39790100) |
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| 摘 要： | 以一套中国春-长穗偃麦草二体异附加系与二体异代换系为材料,用等电聚焦(IEF)研究长穗偃麦草基因组中酸性磷酸酶(AcPh)与碱性磷酸酶(APH)编码基因的染色体定位。结果表明,AcPh大多聚焦于pH5～7范围内,其编码基因位于3E染色体,而APH编码基因则位于4E染色体。由于5E染色体的附加,AcPh活性带强度显著减弱。
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| 关 键 词： | 长穗偃麦草 二体异附加系 二体异代换系 酸性磷酸酶 碱性磷酸酶 基因定位 IEF |
Chromosomal Location of the Genes Coding for Acid Phosphatase and Alkaline Phosphatase in Agropyron elongatum (2n=2x=14, EE) |
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| LI Yu-jing LI Bin LIU Jian-zhong LI Ji-yun YAO Shu-jiang LI Zhen-sheng.Chromosomal Location of the Genes Coding for Acid Phosphatase and Alkaline Phosphatase in Agropyron elongatum (2n=2x=14, EE)[J].Journal of Genetics and Genomics,1998,25(5):449-453. |
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| Authors: | LI Yu-jing LI Bin LIU Jian-zhong LI Ji-yun YAO Shu-jiang LI Zhen-sheng |
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| Institution: | LI Yu-jing~1 LI Bin~1 LIU Jian-zhong~1 LI Ji-yun~2 YAO Shu-jiang~1 LI Zhen-sheng~1 (1 Institute of Genetics the Chinese Academy of Sciences Beijing 100101) (2 R(?)search Center for Eco-environment Chinese Academy of Sciences Beijing 100085) |
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| Abstract: | Chromosomal location of the genes coding for acid phosphatase (AcPh) and alkaline phosphatase (APH) in Agropyron elongatum (2x=2x=14, EE) was conducted using a set of alien disomic addition and substitution lines of Chinese Spimg-Agropyron elongatum. The results indicated that the genes coding for endosperm AcPh was located on chromosome 3E, and these coding for endosperm APH on chromosome 4E. In addition, the activity bands of AcPh in DA5E was faintly due to the addition of chromosome 5E. |
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| Keywords: | Agropyron elongatum Alien disomic addition lines Disomic substitution lines Acid phosphatase Alkaline phosphatase IEF Chromosomal location |
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