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Iron-sulfur cluster assembly: NifU-directed activation of the nitrogenase Fe protein
Authors:Dos Santos Patricia C  Smith Archer D  Frazzon Jeverson  Cash Valerie L  Johnson Michael K  Dean Dennis R
Institution:Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, USA.
Abstract:The NifU protein is a homodimer that is proposed to provide a molecular scaffold for the assembly of Fe-S] clusters uniquely destined for the maturation of the nitrogenase catalytic components. There are three domains contained within NifU, with the N-terminal domain exhibiting a high degree of primary sequence similarity to a related family of Fe-S] cluster biosynthetic scaffolds designated IscU. The C-terminal domain of NifU exhibits sequence similarity to a second family of proposed Fe-S] cluster biosynthetic scaffolds designated Nfu. Genetic experiments described here involving amino acid substitutions within the N-terminal and C-terminal domains of NifU indicate that both domains can separately participate in nitrogenase-specific Fe-S] cluster formation, although the N-terminal domain appears to have the dominant function. These in vivo experiments were supported by in vitro Fe-S] cluster assembly and transfer experiments involving the activation of an apo-form of the nitrogenase Fe protein.
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