Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice |
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Authors: | Qi Yaocheng Wang Hongjuan Zou Yu Liu Cheng Liu Yanqi Wang Ying Zhang Wei |
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Affiliation: | College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China |
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Abstract: | In this study, we identified and functionally characterized the mitochondrial heat shock protein 70 (mtHsp70). Over-expression of mtHsp70 suppressed heat- and H2O2-induced programmed cell death (PCD) in rice protoplasts, as reflected by higher cell viability, decreased DNA laddering and chromatin condensation. Mitochondrial membrane potential (Δψm) after heat shock was destroyed gradually in protoplasts, but mtHsp70 over-expression showed higher Δψm relative to the vector control cells, and partially inhibited cytochrome c release from mitochondria to cytosol. Heat treatment also significantly increased reactive oxygen species (ROS) generation, a phenomenon not observed in protoplasts over-expressing mtHsp70. Together, these results suggest that mtHsp70 may suppress PCD in rice protoplasts by maintaining mitochondrial Δψm and inhibiting the amplification of ROS. |
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Keywords: | Abbreviations: DAPI, 4&prime ,6-diamidino-2-phenylindole Δψm, mitochondrial membrane potential H2DCFDA, 2&prime ,7&prime -dichlorodihydrofluoresceindiacetate JC-1, 5,5&prime ,6,6&prime -tetrachloro-1,1&prime ,3,3&prime -tetraethyl-imidacarbocyanine iodide mtHsp70, mitochondrial heat shock protein 70 PCD, programmed cell death ROS, reactive oxygen species |
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