Cyanobacterial phytochrome Cph2 is a negative regulator in phototaxis toward UV-A |
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| Authors: | Moon Yoon-Jung Kim Soo Youn Jung Kwang-Hwan Choi Jong-Soon Park Young Mok Chung Young-Ho |
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| Affiliation: | aDivision of Life Science, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea;bDepartment of Life Science, Sogang University, Shinsu-Dong 1, Mapo-Gu, Seoul 121-742, Republic of Korea;cGraduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Republic of Korea;dMass Spectrometry Research Center, Korea Basic Science Institute, 804-1 Yangchung-Ri, Ochang, Chungbuk 363-883, Republic of Korea |
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| Abstract: | We investigated the wavelength dependence and photon-fluence rate response relationship for phototaxis of wild-type and a cyanobacterial phytochrome 2 (cph2) mutant in cyanobacterium Synechocystis sp. PCC 6803. Compared to wild-type, the cph2 mutant exhibited maximal activity for positive phototaxis at the near-UV spectral range. Two cysteine to serine substitutions in two chromophore-binding domains showed a similar cph2 mutant phenotype under UV-A. Epistasis of a pixJ mutation over a cph2 mutation implied that pixJ gene acts downstream of the cph2 gene with respect to UV-A-induced positive phototaxis. Therefore, we suggest that Cph2 is essential for the inhibition of positive phototaxis toward UV-A. |
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| Keywords: | Abbreviations: GAF, cGMP-phosphodiesterase, adenylate cyclase, FhlA PCB, phycocyanobilin SyCikA, circadian input kinase homolog of Synechocystis sp. PCC 6803 |
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