Expression of S100A8/A9 in HaCaT keratinocytes alters the rate of cell proliferation and differentiation |
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| Authors: | Voss Andreas Bode Günther Sopalla Claudia Benedyk Malgorzata Varga Georg Böhm Markus Nacken Wolfgang Kerkhoff Claus |
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| Institution: | aInstitute of Immunology, University of Muenster, Germany;bDepartment of Dermatology, University of Muenster, Germany;cInstitute for Molecular Virology, ZMBE, University of Muenster, Germany |
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| Abstract: | S100A8/A9 promotes NADPH oxidase in HaCaT keratinocytes and subsequently increases NFκB activation, which plays important roles in the balance between epidermal growth and differentiation. S100A8/A9-positive HaCaT cells present with a significantly reduced rate of cell division and greater expression of two keratinocyte differentiation markers, involucrin and filaggrin, than control cells. S100A8/A9 mutants fail to enhance NFκB activation, TNFα-induced IL-8 gene expression and NFκB p65 phosphorylation, and S100A8/A9-positive cells demonstrate better cell survival in forced suspension culture than mutant cells. S100A8/A9 is induced in epithelial cells in response to stress. Therefore, S100A8/A9-mediated growth arrest could have implications for tissue remodeling and repair. |
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| Keywords: | Abbreviations: AA arachidonic acid CFDA carboxy-fluorescein diacetate succinimidyl ester FACS fluorescence-activated cell sorting ROS reactive oxygen species |
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