Removal of the sphingolipid impurity from preparations of yeast phosphatidyl inositol

A complex sphingolipid containing inositol and mannose, present in lipid extracted from toluene-autolyzed baker's yeast, was eluted from silicic acid columns immediately after phosphatidyl inositol, and was the main nitrogenous impurity in crude preparations of this phospholipid. Nitrogenfree phosphatidyl inositol was obtained by rechromatography on alumina. Modifications to the chromatographic procedure also gave diphosphatidyl glycerol containing the theoretical 4.29% P.