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| 内皮型一氧化氮合酶在胚胎小鼠肾脏发育中的表达 | |
| 引用本文： | 包翠芬,刘霞,王晓梅,穆长征. 内皮型一氧化氮合酶在胚胎小鼠肾脏发育中的表达[J]. 中国组织化学与细胞化学杂志, 2008, 17(2): 196-199. DOI: 10.3870/j.issn.1004-1850.2008.02.016 |
| 作者姓名： | 包翠芬 刘霞 王晓梅 穆长征 |
| 作者单位： | 1. 辽宁医学院科学实验中心,锦州,121001 2. 组织胚胎学教研室,锦州,121001 3. 附属第一医院,锦州,121001 |
| 摘 要： | 目的观察妊娠不同时期胚胎小鼠肾脏发育不同阶段内皮型一氧化氮合酶（eNOS）的表达，探讨eNOS在胚胎小鼠肾脏早期发育中的意义。方法分别取胚龄13d（E13）、14d（E14）、15d（E15）、16d（E16）、18d（E18）组及新生组（P0）小鼠各10只，共6组。分别用免疫组化及免疫印迹方法对小鼠肾脏内eNOS表达进行定性、定量分析。结果（1）免疫组化结果显示：E14、E15组eNOS在生肾区呈阳性表达；E16组生肾区表达减弱，肾近端小管呈强阳性表达，同时远端小管及肾脏小动脉内皮也有阳性表达；E18组、P0组近端小管呈强阳性表达，远端小管呈阳性表达，髓质中的集合管eNOS表达弱阳性，而致密斑呈阴性表达。（2）免疫印迹结果显示：E14组肾脏eNOS含量较少，随后逐渐增多，PD0组eNOS含量最多。结论（1）eNOS在小鼠肾脏第14d开始呈阳性表达，以后含量逐渐升高，出生时含量最高。（2）eNOS表达部位从生肾区开始，以后其表达逐渐减弱甚至消失，而肾近端小管、远端小管的表达晚于生肾区，且呈逐渐增强趋势，至出生时达到最强。这一结果表明eNOS在胚胎小鼠肾脏发育的早期阶段起重要调节作用。 |
| 关 键 词： | 内皮型一氧化氮合酶 小鼠 肾 发育 |
| 修稿时间： | 2007-07-02 |
EXPRESSION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE IN MOUSE KIDNEY DURING FETAL DEVELOPMENT |
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| Bao Cuifen,Liu Xia,Wang Xiaomei,Mu Changzheng. EXPRESSION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE IN MOUSE KIDNEY DURING FETAL DEVELOPMENT[J]. Chinese Journal of Histochemistry and Cytochemistry, 2008, 17(2): 196-199. DOI: 10.3870/j.issn.1004-1850.2008.02.016 | |
| Authors: | Bao Cuifen Liu Xia Wang Xiaomei Mu Changzheng |
| Affiliation: | Bao Cuifen,liu Xia1,Wang Xiaomei2,Mu Changzheng1(Centre of Scientific Expreiment,1Department of Histology , Embryology,2The First Affiliated Hospital,Liaoning Medical College,Jinzhou 121001,China) |
| Abstract: | Objective To investigate the expression of endothelial nitric oxide synthase(eNOS)in fetal mouse kidney at different embryonic stages,and discuss the role of eNOS in mouse kidney during fetal development.Methods Kunming mice were divided into six groups at embryonic day 13(E13),14(E14),15(E15),16(E16),18(E18)and birth(P0),with 10 mice each.The intrarenal eNOS localization was detected by immunohistochemistry.Renal eNOS content was measured by Western blot.Results(1)eNOS was identified in the nephrogenic zon... |
| Keywords: | Endothelial nitric oxide synthase Fetal mouse Kidney Development |
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