抗感白粉病青稞的内生真菌群落差异分析

种子内生微生物组与种子的健康发育有着密切的关系。本研究收集对白粉病抗病和易感共11个品种的青稞种子，利用扩增子测序技术对内生真菌的群落、丰度和多样性进行研究。从不同品种的青稞种子中共鉴定4个门、17个纲、35个目、66个科、107个属的内生真菌，其中子囊菌门Ascomycota、担子菌门Basidiomycota和被孢菌门Mortierellomycota是优势菌门；链格孢属Alternaria、微座孢属Microdochium、亚隔孢壳属Didymella、镰刀菌属Fusarium和球腔菌属Mycosphaerella为优势菌属。根据生物信息学注释结果，在鉴定到的107个属中，抗病品种的青稞种子中特有的属有20个，感病品种的青稞种子中特有的属有50个；通过对两组样本的分析，发现感病品种中真菌物种多样性高于抗病品种。本研究通过扩增子测序技术初步确定了青稞抗、感白粉病两组样品中的真菌群落结构和优势菌群，为进一步研究青稞内携真菌与抗/感病的关系提供了基础，从内生菌的角度为青稞白粉病的防控提供新的策略。

Difference of endophytic fungal communities between highland barley cultivars resistant and susceptible to powdery mildew

The endophytic microbiome in seeds is closely related to the healthy development of seeds. In this study, the seeds of 11 highland barley varieties resistant or susceptible to powdery mildew were collected and subjected to amplicon sequencing to investigate the community, abundance and diversity of endophytic fungi. Endophytic fungi belonging to 4 phyla, 17 classes, 35 orders, 66 families and 107 genera were identified. Ascomycota, Basidiomycota and Mortierellomycota were the dominant phyla. Alternaria, Microdochium, Didymella Fusarium and Mycosphaerella were the dominant genera. The results of bioinformatics annotation show that among the 107 genera identified, 20 are specific in the seeds of resistant varieties and 50 are specific in the seeds of susceptible varieties. The fungal species diversity of susceptible cultivars was higher than that of resistant cultivars. This study provides a basis for further study on the relationship between endophytic fungi and resistance or susceptibility of highland barley to powdery mildew. It is hopeful to develop new strategies for disease prevention and control.